氯霉素检测
发布时间：2021-10-23 18:07:01 点击:0
氯霉素快速检测试剂盒说明书
一、氯霉素检测原理
本试剂盒采用间接竞争ELISA方法，在微孔条上预包被偶联抗原，样本中的残留物氯霉素将和微孔条上预包被的偶联抗原竞争抗氯霉素抗体，加入酶标记物后，用TMB底物显色，样本吸光值与其所含残留物氯霉素的含量成负相关，与标准曲线比较即可得出相应残留物氯霉素的含量。
二、氯霉素检测试剂盒特性
试剂盒灵敏度：0.05ppb
u孵育温度：25℃
u孵育时间：30min～30min～15min
样本检测下限
蛋类、牛奶（方法一）·····························0.1ppb尿液、血清、肠衣、饲料、奶粉···················0.05ppb组织、肝脏、蜂蜜、牛奶（方法二）·············0.025ppb
交叉反应率
氯霉素········································100%
甲砜霉素······································<0.1%
氟甲砜霉素······································<0.1%
样本回收率
组织、肝脏································
85%±20%
蜂蜜、肠衣································
83%±25%
牛奶、饲料································
75%±23%
尿液、血清································
70%±18%
三、试剂盒组成
1
微量测试孔
每条8孔，一板12条
标准液×6瓶
0ppb
0.05ppb
2
0.15ppb
0.45ppb
（1ml/瓶）
1.35ppb
4.05ppb
3
酶标记物
12ml
红色帽
4
抗体浓缩液
1ml
蓝色帽
5
底物A液
7ml
白色帽
6
底物B液
7ml
黑色帽
7
终止液
7ml
黄色帽
8
20X浓缩洗涤液
40ml
白色帽
9
2X浓缩复溶液
50ml
透明帽
https://www.chem17.com/st195854/product_32014246.html
http://www.elisafx.com/fengxiang02-SonList-1870755/