Biotinylated Anti-人Leptin PeproTech

北京方程生物经营Biotinylated Anti-人Leptin PeproTech产品，欢迎来电咨询！ 

订购热线：010-57266903 

Biotinylated Anti-人Leptin PeproTech2.　加样：加一定稀释的待检样品0.1ml于上述已包被之反应孔中，置37℃ 孵育1小时.然后洗涤.（同时做空白孔，阴性对照孔及阳性对照孔）产品是我们的优势产品，质量可靠，性能稳定Biotinylated Anti-人Leptin PeproTech细胞中如何防止黑点生成? (1) 尽可能地冻融. (2) 基无需37℃水浴. (3) 基保持佳PH值7.0- 7.2. (4) 严格控制配制基的水质，容器定期刷洗. (5) 细胞传代的佳，细胞切勿生长过老.每孔加3H-TdR 0.5μCi/50μl ↓10h 用多头细胞收集仪收获于玻璃纤维滤纸上 ↓ 干燥后移入液闪瓶中，加入1ml闪烁液，于液闪仪中测β射线的cpm值. 计算： 1. 根据cpm值，将能使7TD1细胞3H-TdR掺入cpm值达到大值50%的IL-6的活性定为1u/ml. 2. 以指数(SI)表示. ㈢ 试剂和器材 1. IL-6依赖的7TD1细胞系 2. 10%FCS-RPMI1640及无FCS的RPMI1640 3. 3H-TdR 4. IL-6 5. PPO,POPOP,. 6. 9999型玻璃纤维滤纸. 7. 96孔平底板 8. 多头细胞收集仪 9. CO2孵箱 10. β液闪计数仪

Biotinylated Anti-人Leptin PeproTech

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