重组人soluble TRAIL Receptor-1 PeproTech

北京方程生物经营重组人soluble TRAIL Receptor-1 PeproTech产品，欢迎来电咨询！ 

订购热线：010-57266903 

重组人soluble TRAIL Receptor-1 PeproTech细胞中出现黑点是污染吗?如何处理? 一旦您在细胞的中发现有黑点生成，首先，要观察基是否混浊，然后，在镜下观察细胞生长的状态，黑点是否.如果细胞被污染，微生物则会大量繁殖，基就会迅速变黄、变混浊.污染包括污染、支原体污染等. 如果在镜下观察细胞，生长状态良好，与黑点出现前相比，没有任何变化，那么，黑点的出现可能与以下几种情况有关： (1)细胞生长过老，破碎的细胞残骸; (2)不好，反复冻融的结果; (3)配制基的pH值偏高，不宜细胞生长; (4)配制基的水质、容器不合格.可应用离心、过滤的去除这些黑点; (5)原代细胞中出现小黑点，可能是原代组织中的杂质，多次传代可以.产品是我们的优势产品，质量可靠，性能稳定重组人soluble TRAIL Receptor-1 PeproTechDescription : Recombinant Human BMP-4 produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 116 amino acids and having a molecular mass of 13009 Dalton. BMP-4 is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: BMP-4 was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives. Solubility: It is recommended to reconstitute the lyophilized BMP-4 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability: Lyophilized BMP-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution BMP-4 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Lys-His-His. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Applications: 1.Molecular standard (Western, ELISA) in studying secreted BMP-4 2.Preparing antibodies for BMP-4 monomer. 3.Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.胎牛血清功能 牛血清是细胞培养中用量**的天然培养基，含有丰富的细胞生长必须的营养成份，常用于动物细胞的体外培养，具有极为重要的功能. 1.提供对维持细胞指数生长的**，基础培养基中没有或量很少的营养物，以及主要的低分子营养物. 2. 提供结合蛋白，能识别维生素、脂类、金属和其他**等，能结合或调变它们所结合的物质活力. 3.有些情况下结合蛋白质能与有毒金属和热原质结合，起到**作用. 4.是细胞贴壁、铺展在塑料培养基质上所需因子来源. 5.起酸碱度缓冲液作用. 6.提供蛋白酶抑制剂，使在细胞传代时使剩余胰蛋白酶失活，保护细胞不受伤害.

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