Anti-Murine VEGF PeproTech

北京方程生物经营Anti-Murine VEGF PeproTech产品，欢迎来电咨询！ 

订购热线：010-57266903 

Anti-Murine VEGF PeproTech每孔加3H-TdR 0.5μCi/50μl ↓10h 用多头细胞收集仪收获于玻璃纤维滤纸上 ↓ 干燥后移入液闪瓶中，加入1ml闪烁液，于液闪仪中测β射线的cpm值. 计算： 1. 根据cpm值，将能使7TD1细胞3H-TdR掺入cpm值达到大值50%的IL-6的活性定为1u/ml. 2. 以指数(SI)表示. ㈢ 试剂和器材 1. IL-6依赖的7TD1细胞系 2. 10%FCS-RPMI1640及无FCS的RPMI1640 3. 3H-TdR 4. IL-6 5. PPO,POPOP,. 6. 9999型玻璃纤维滤纸. 7. 96孔平底板 8. 多头细胞收集仪 9. CO2孵箱 10. β液闪计数仪产品是我们的优势产品，质量可靠，性能稳定Anti-Murine VEGF PeproTechIf my FBS arrives partially thawed, can I still use it? Our studies have shown that short-term storage of thawed FBS at 4 degrees C for up to 28 days causes no decrease in growth or viability performance. FBS stored at 4 degrees C for longer periods of time should be evaluated on a case-by-case basis should this storage condition be necessary按如下操作可避免沉淀的产生： (1)解冻时，请随时将之摇晃均匀，使温度及成分均一，沉淀的发生. (2) 分装冻存时，须规则摇晃均匀(小心勿造成气泡)，使温度与成分均一. (3) 勿直接由-20℃直接至37℃解冻，因温度改变太大，容易造嘲白质凝结而发生沉淀. (4) 勿将置于37℃太久，否则会浑浊，同时中许多较不的成份也会因此受到，从而影响的品质. (5) 的热灭活非常容易造成沉淀物的增多，若非必要，可以无须做此步骤. (6) 若必须做的热灭活，请遵守56℃，30分钟的原则，并且随时摇晃均匀.温度过高，时间过久或摇晃不均匀，都会造成沉淀物的增多.

Anti-Murine VEGF PeproTech

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