重组人FGF-acidic PeproTech

北京方程生物经营重组人FGF-acidic PeproTech产品，欢迎来电咨询！ 

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重组人FGF-acidic PeproTechDescription : Recombinant Human BMP-4 produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 116 amino acids and having a molecular mass of 13009 Dalton. BMP-4 is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: BMP-4 was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives. Solubility: It is recommended to reconstitute the lyophilized BMP-4 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability: Lyophilized BMP-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution BMP-4 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Lys-His-His. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Applications: 1.Molecular standard (Western, ELISA) in studying secreted BMP-4 2.Preparing antibodies for BMP-4 monomer. 3.Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.产品是我们的优势产品，质量可靠，性能稳定重组人FGF-acidic PeproTech6.　结果判定：可于白色背景上，直接用观察结果：反应孔内颜色越深，阳性程度越强，阴性反应为无色或极浅，依据所呈颜色的深浅，以"+"、"-"号表示.也可测OD值：在ELISA检测仪上，于450nm（若以ABTS显色，则410nm）处，以空白对照孔调零后测各孔OD值，若大于规定的阴性对照OD值的2.1倍，即为阳性. 白细胞介素6(IL-6)是一种具有多重免疫 调节功能的细胞因子.HTLV-1感染的T淋巴母细胞系、单核细胞、血管内皮细胞、成纤维细胞以及某些细胞均可产生IL-6.某些急性炎症、自身免疫性、淋巴细胞恶性(如多发性瘤)等与病人IL-6水平异常有关. ㈠ 原理：IL-6在体外能促进B细胞杂交瘤的生长，故又称为杂交瘤生长因子(HGF).小鼠B细胞杂交瘤7TD1是一株对IL-6依赖的细胞系，由Van Snick 1986年建立，只有在IL-6存在的基中才能生长，可以作为指示细胞来测定待检样品中IL-6水平.这类IL-6依赖的小鼠B细胞杂交瘤细胞系还有MH60?BSF2、B9等. ㈡ ：主要为3H-TdR掺入法 IL-6依赖的7TD1细胞用无FCS的RPMI1640洗涤2次 每次1000rpm，5min，除去原液中的IL-6来源宿主 大肠杆菌中重组表达 序列 The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Pro-Ile-Arg. 溶解建议 提供的小鼠白介素-1β （mIL 1 beta）产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含小鼠白介素-1β （mIL 1 beta）的溶液（不含添加剂）中冻干产生的.我们建议加无菌的18MΩ-cm 水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释. 保存建议 小鼠白介素-1β （mIL 1 beta）冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存.重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存.请避免反复冻融.

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