重组大鼠 RANTES (CCL5) PeproTech
北京方程生物经营重组大鼠 RANTES (CCL5) PeproTech产品,欢迎来电咨询!
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重组大鼠 RANTES (CCL5) PeproTech3. 加酶标:于各反应孔中,加入新鲜稀释的酶标(经滴定后的稀释度)0.1ml.37℃ 孵育0.5~1小时,洗涤. 4. 加底物液显色:于各反应孔中加入临时配制的TMB底物溶液0.1ml,37℃ 10~30分钟. 5. 终止反应:于各反应孔中加入2M硫酸0.05ml.产品是我们的优势产品,质量可靠,性能稳定重组大鼠 RANTES (CCL5) PeproTech2.可检测动物类型丰富:人、猴、大鼠、小鼠、豚鼠、兔、猪、犬、牛、绵羊、鸡、虾、鱼等 3.可检测指标齐全:白介素,干扰素,血管紧张素,肺炎衣原体,趋化因子、生长因子基质金属蛋白酶、炎症因子、血管生成素 等等 指标Description : Recombinant Human BMP-4 produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 116 amino acids and having a molecular mass of 13009 Dalton. BMP-4 is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: BMP-4 was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives. Solubility: It is recommended to reconstitute the lyophilized BMP-4 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability: Lyophilized BMP-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution BMP-4 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Lys-His-His. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Applications: 1.Molecular standard (Western, ELISA) in studying secreted BMP-4 2.Preparing antibodies for BMP-4 monomer. 3.Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.
重组大鼠 RANTES (CCL5) PeproTech
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