pRSET-ECFP大肠青色荧光表达质粒_供应_中贸商网-贸易商务资源网

pRSET-ECFP大肠青色荧光表达质粒

发布时间:2018-06-20 11:08:08 产品编号:GY-5-194284684 分享

价格:未填
品牌:pRSET-ECFP大肠青色荧光表达质粒
发货:2天内
信息标签:pRSET-ECFP大肠青色荧光表达质粒,供应,医药与器械,生物制品

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pRSET-ECFP大肠青色荧光表达质粒

使用说明：

我公司的各批次产品质粒菌株均经过严格的多重验证，如存在存在质量问题，请及时通知我们退换，有其他问题都可随时联系我们，诚信质优服务于每一个**客户是我们的宗旨。

产品信息

名称	规格
pRSET-ECFP大肠青色荧光表达质粒	2μg质粒干粉

基本信息

质粒分类：大肠杆菌载体；蛋白表达质粒

原核抗性：氨苄青霉素Amp

筛选标记：青色荧光蛋白ECFP

克隆菌株：大肠杆菌DH5α

培养条件：37℃

质粒简介

pRSET-ECFP是一个大肠杆菌青色荧光表达质粒。

质粒图谱

质粒序列

LOCUS Exported 3600 bp ds-DNA circular SYN 30-SEP-2016

DEFINITION synthetic circular DNA

FEATURES Location/Qualifiers

source 1..3600

/organism="synthetic DNA construct"

/mol_type="other DNA"

promoter 9..27

/note="T7 promoter"

/note="promoter for bacteriophage T7 RNA polymerase"

RBS 59..81

/note="efficient ribosome binding site from bacteriophage

T7 gene 10 (Olins and Rangwala, 1989)"

CDS 101..118

/codon_start=1

/product="6xHis affinity tag"

/note="6xHis"

/translation="HHHHHH"

CDS 122..154

/codon_start=1

/product="leader peptide from bacteriophage T7 gene 10"

/note="T7 tag (gene 10 leader)"

/note="promotes efficient translation in E. coli"

/translation="MASMTGGQQMG"

CDS 158..181

/codon_start=1

/product="Xpress(TM) epitope tag, including an enterokinase

recognition and cleavage site"

/note="Xpress(TM) tag"

/translation="DLYDDDDK"

CDS 209..928

/codon_start=1

/product="enhanced CFP"

/note="ECFP"

/note="mammalian codon-optimized"

/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK

ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

EFVTAAGITLGMDELYK"

terminator 1004..1051

/note="T7 terminator"

/note="transcription terminator for bacteriophage T7 RNA

polymerase"

rep_origin 1148..1603

/direction=RIGHT

/note="f1 ori"

/note="f1 bacteriophage origin of replication; arrow

indicates direction of (+) strand synthesis"

promoter 1629..1733

/gene="bla"

/note="AmpR promoter"

CDS 1734..2594

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/note="AmpR"

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

rep_origin 2765..3353

/direction=RIGHT

/note="ori"

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

ORIGIN

1 cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta gaaataattt

61 tgtttaactt taagaaggag atatacatat gcggggttct catcatcatc atcatcatgg

121 tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa

181 ggatcgatgg ggatccgaat tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg

241 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc

301 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac

361 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg

421 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga

481 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc

541 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt

601 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt

661 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa

721 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga

781 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga

841 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac

901 tctcggcatg gacgagctgt acaagtaact cgagaagctt gatccggctg ctaacaaagc

961 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg

1021 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatctgg

1081 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc

1141 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc

1201 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt

1261 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc

1321 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt

1381 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt

1441 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt

1501 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa

1561 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg

1621 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc

1681 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta

1741 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg

1801 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg

1861 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac

1921 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg

1981 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt

2041 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg

2101 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac

2161 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt

2221 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag

2281 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc

2341 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc

2401 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta

2461 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg

2521 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga

2581 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac

2641 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa

2701 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat

2761 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc

2821 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg

2881 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc

2941 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg

3001 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg

3061 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa

3121 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg

3181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga

3241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct

3301 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca

3361 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc

3421 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg

3481 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc

3541 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagg atctcgatcc

//

联系方式

公司: 武汉景秀科研生物科技有限公司
状态:离线发送信件
姓名:刘经理(先生)
职位:经理
电话:159-0712-6438
手机:13971316819
地区:湖北
地址:湖北省武汉市武昌区民主路北789号

QQ:1206403291
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