质粒序列
LOCUS pKC1139 6573 bp DNA circular 14-MAR-2012
FEATURES Location/Qualifiers
CDS complement(167..508)
/vntifkey="4"
/label=lacZa
CDS 1730..2503
/vntifkey="4"
/label=aac(3)IV
primer complement(509..525)
/vntifkey="27"
/label=sp6
primer complement(528..547)
/vntifkey="27"
/label=seq-152-anti
misc_feature 765..934
/vntifkey="21"
/label=ori-PUC18
misc_feature 6050..6161
/vntifkey="21"
/label=oriT
CDS complement(3443..4885)
/vntifkey="4"
/label=Rep-pSG5
promoter complement(4855..5273)
/vntifkey="30"
/label=promoter
promoter 1662..1711
/vntifkey="30"
/label=promoter
base COUNT 1180 a 2002 c 2082 g 1309 t
ORIGIN
1 gatctacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
61 agctctcgca gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
121 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt gatcggcact
181 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattt atgcggtgtg
241 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
301 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
361 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
421 gttgtaaaac gacggccagt gccaagcttg ggctgcaggt cgactctaga ggatccgcgg
481 ccgcgcgcga tatcgaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc
541 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
601 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
661 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
721 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
781 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
841 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
901 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
961 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
1021 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
1081 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
1141 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
1201 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
1261 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
1321 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
1381 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
1441 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
1501 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
1561 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg gttcatgtgc
1621 agctccatca gcaaaagggg atgataagtt tatcaccacc gactatttgc aacagtgccg
1681 ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag tgcaatgtcg tgcaatacga
1741 atggcgaaaa gccgagctca tcggtcagct tctcaacctt ggggttaccc ccggcggtgt
1801 gctgctggtc cacagctcct tccgtagcgt ccggcccctc gaagatgggc cacttggact
1861 gatcgaggcc ctgcgtgctg cgctgggtcc gggagggacg ctcgtcatgc cctcgtggtc
1921 aggtctggac gacgagccgt tcgatcctgc cacgtcgccc gttacaccgg accttggagt
1981 tgtctctgac acattctggc gcctgccaaa tgtaaagcgc agcgcccatc catttgcctt
2041 tgcggcagcg gggccacagg cagagcagat catctctgat ccattgcccc tgccacctca
2101 ctcgcctgca agcccggtcg cccgtgtcca tgaactcgat gggcaggtac ttctcctcgg
2161 cgtgggacac gatgccaaca cgacgctgca tcttgccgag ttgatggcaa aggttcccta
2221 tggggtgccg agacactgca ccattcttca ggatggcaag ttggtacgcg tcgattatct
2281 cgagaatgac cactgctgtg agcgctttgc cttggcggac aggtggctca aggagaagag
2341 ccttcagaag gaaggtccag tcggtcatgc ctttgctcgg ttgatccgct cccgcgacat
2401 tgtggcgaca gccctgggtc aactgggccg agatccgttg atcttcctgc atccgccaga
2461 ggcgggatgc gaagaatgcg atgccgctcg ccagtcgatt ggctgagctc atgagcggag
2521 aacgagatga cgttggaggg gcaaggtcgc gctgattgct ggggcaacac gtggagcgga
2581 tcggggattg tctttcttca gctcgctgat gatatgctga cgctcaatgc cgtttggcct
2641 ccgactaacg aaaatcccgc atttggacgg ctgatccgat tggcacggcg gacggcgaat
2701 ggcggagcag acgctcgtcc gggggcaatg agatatgaaa aagcctgaac tcaccgcgac
2761 gtatcgatgt cgaggttcct caggggagcc accccagaga agccctcgga gctgagcgga
2821 gctatttcca aagccatgcc agctagagac agtgcacacc gccaagcatc tgcaaaaccc
2881 tcgcctggag ggaaagtgca atgtacgtac gctggtttcc ctccagaaag ggattctgcg
2941 gcttctcaac tttggaagaa gaggcggaac gaactgctgt ttcagcctac tcatgtgagg
3001 aggctggtcc tttaccctga tgcccgaggg catcagggta aaggaccagc ctcgccaact
3061 aggagcgcta ccaaggcgaa cacaacccag gacttggtag aacccttcgc cggagaagtt
3121 cagactcatc ggcatgagca cccctcgccg cgcactcggc accggtccgt cgaccaccac
3181 caggacgtcg ttgtcgacgt cggccccgcg gctcctgccc gccgaacgcg tcgtcgtcga
3241 cggcctggtg ctcatcgacg agcacccgga gccaggtgaa aagcgccggc ggacgctcgg
3301 actgggcgcg ggattccagc agtaacccag gtccgccggc cacctcacgg caggcagacc
3361 ctcggctttc gcgccgggac cgccatgaga ccgccacccg gatgtccggg gtggcggtct
3421 catgggcggt ccctcagcgg ccctacgcga ccgctgtgtc gacgcggagg cagtctccgg
3481 ggtgctggcc ccagggctgg agtaccgggg cgagcttgcc cttgcagcgg cggcacacgg
3541 gcgacgcggc gccgggcggg ggagtcttga cgtcgacggt gaccggcttc ggcttcaggc
3601 gcttccgaag gctgatcgtc gggcggatcg cctccttctt cggctgacgc actcggtcga
3661 gcgaagcggc cagctcctcc gtgcgggcct cgttggcctt ccggcgcgcc tcgcggaaag
3721 cagcttcctc ctcggacatg acctcgaacc tcatctggtc agcgtcaagg tcgcccggcg
3781 cggccggcgc ttccggggcg ggcgggtcca ggacgtcctt gccccacacc aggccccagg
3841 actcgacgag ccgccggacg cccggtaggc cgtacgtctc ggcgaccttg atgaggtcga
3901 ggcgacgtcc ggcgacgcgg gcgatgtatc ggtaccagat gtaggccggg atgaccgcga
3961 tggcgaccag gccctcggtg tcgtcggtga tctcctcctc ggtgcggacg tcctgctgga
4021 tgccgagttc cttgatcagc cggttcaggt tctgcgaccg gtagtgcttg cggacctgga
4081 agacgccgaa ctcgcgctcg cggtacttct cgacgaacgg gccgggccga cgaagccgct
4141 gcagctcggc ggccgccgcg tcgcccaggt cgagcggtcc catgcggtcg tcgccgcgac
4201 cggccttgaa gttctgtccg gccagctcca ggccgatctt ggcgacgccg cccttggtct
4261 tgtcgccgtc cttgtagagg tagcgggcct gcttgcccgc atcgccgtca gcggcgtccg
4321 cgccgttgag tgggcgcacg tcggtgccgt ggcccttgcc ctcacaggag caaccgggcc
4381 ggtcgcacgt ctcgctgacg gtgtagccgc ccgcggattc gaccccggcg gcccaggctc
4441 cggcgagtgc gtcgcggaac gcggcctggg cgtccgggcc gagcacctcg cgggtgaccc
4501 agagcgtgtg ccagtgcagg tgccagccgg agccccagcc gaaggtgtcc tcgaaggccc
4561 gctcgtagcc gatgatcccg aagtcgtcgc gcatcgtgcg ccagcggcgg ccggacgagc
4621 cgtacgcgcc cttccagccg tcgtgcaaga ccgcgaccag gccgtgccgc attcccttgc
4681 ggacggtgcc gaacgccatg cgctcgaagt ggcgcaacgt gttcgtgcca aggtgcagcc
4741 cgtacccggc gtccgcgaga ccgtcggcgg cgagctgcac gttcgagccc cgtacggcca
4801 ggatgcggct catgcaccac gggcaggtgt ggacgttgtt gcagcggcac gtgttgcccc
4861 acgtcgcctc gcccggcttc cacatcagct cggccgtccc ggcagtgagc cgggtcccgc
4921 agcccttgaa cgcctcgttc agcgacaccg tctggtgccg gtcccgccgg gcgaaccgct
4981 cgtcgcgcgg gtcctcccgc ccggctgtcg cggcaccctc gtttggggta gaacccgttc
5041 cagttacagc gctctgacct gcagtggacg gagattttcc cttactacta aagcccgcgt
5101 ccggattacc cgctgtagtc gtgcttgcta cgctgcgtga ctggtccgca atgagacgct
5161 ttgcgcgctt tcggcaggcg tccgagcagt agattttggg gcgcttcccg gggatgtgga
5221 cgatcggggt gccgcagtgg cacttcggtc cggcggggcg cggtggtgtc gacgcgctgt
5281 tctctcgtac gctcgtcaca gagcaaacgt cctcactcgg catgctgcgc cggttcgggg
5341 gcggcgagcc cgggaggcca atcccgggct cgtgccattt ctgggtcctg ttgatcctgg
5401 cattggtgtg gccgttcatt gcccctgctc gctcctgacg cgccgataga cgtccgatac
5461 gcccggtgct ggtgggattt gataggtcgg aagaagcccc gcccgggcct gggcggggct
5521 tcctgtgcgt caggacctcc tcgtcgtgag cctcttcggc ctatggacgg agtgacctcg
5581 tgatccgtta cagccgcgcg cgctcgcgta gagcggtctc atcagttcca cgaacggtcc
5641 tcttcgcaga tcagggcgtt ggggcggagt ctcaccaagg actacgtctg ctggcgattt
5701 ccgttacacc ccgggcggtg gccggcgcac acgcgcgccc gcgttgggca gtgcagaaag
5761 tgcagaaacc taggcgctga tggtccaggt ccacggttcg tcgtcggcgg cggcgcgggc
5821 ggcggcgtcg gccagggcgc gggcgagacc ggctacggcg ggcttgatgc gccggttgcg
5881 ggcgaccttg agcagctagt atgcaggtcg acggatcttt tccgctgcat aaccctgctt
5941 cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt
6001 ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat
6061 aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgcacct
6121 ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gcatgagcct gtcggcctac
6181 ctgctggccg tcggccaggg ctacaaaatc acgggcgtcg tggactatga gcacgtccgc
6241 gagctggccc gcatcaatgg cgacctgggc cgcctgggcg gcctgctgaa actctggctc
6301 accgacgacc cgcgcacggc gcggttcggt gatgccacga tcctcgccct gctggcgaag
6361 atcgaagaga agcaggacga gcttggcaag gtcatgatgg gcgtggtccg cccgagggca
6421 gagccatgac ttttttagcc gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt
6481 ccccatgcgc tccatcaaga agagcgactt cgcggagctg gtgaagtaca tcaccgacga
6541 gcaaggcaag accgatcccc ggggacctgc ata
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