人蛋氨酸腺苷转移酶Ⅰα(MAT1α)ELISA测试检测盒

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人蛋氨酸腺苷转移酶Ⅰα(MAT1α)ELISA测试检测盒Description : Recombinant Human BMP-4 produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 116 amino acids and having a molecular mass of 13009 Dalton. BMP-4 is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: BMP-4 was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives. Solubility: It is recommended to reconstitute the lyophilized BMP-4 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability: Lyophilized BMP-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution BMP-4 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Lys-His-His. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Applications: 1.Molecular standard (Western, ELISA) in studying secreted BMP-4 2.Preparing antibodies for BMP-4 monomer. 3.Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.产品是我们的优势产品，质量可靠，性能稳定人蛋氨酸腺苷转移酶Ⅰα(MAT1α)ELISA测试检测盒● 抗原亲和纯化（APP）的多克隆抗体 PeproTech所有的多克隆抗体均经抗原亲和纯化，因此可保证抗体的高度特异性3.　加酶标：于各反应孔中，加入新鲜稀释的酶标（经滴定后的稀释度）0.1ml.37℃ 孵育0.5～1小时，洗涤. 4.　加底物液显色：于各反应孔中加入临时配制的TMB底物溶液0.1ml，37℃ 10～30分钟. 5.　终止反应：于各反应孔中加入2M硫酸0.05ml.

人蛋氨酸腺苷转移酶Ⅰα(MAT1α)ELISA测试检测盒

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