猪载脂蛋白A1(APOA1)ELISA检测盒

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猪载脂蛋白A1(APOA1)ELISA检测盒实验标准品图： 40 ng/L 5号标准品 150μl的原倍标准品加入150μl标准品稀释液 20 ng/L 4号标准品 150μl的5号标准品加入150μl标准品稀释液 10 ng/L 3号标准品 150μl的4号标准品加入150μl标准品稀释液 5 ng/L 2号标准品 150μl的3号标准品加入150μl标准品稀释液 2.5 ng/L 1号标准品 150μl的2号标准品加入150μl标准品稀释液产品是我们的优势产品，质量可靠，性能稳定猪载脂蛋白A1(APOA1)ELISA检测盒Description : Recombinant Human BMP-4 produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 116 amino acids and having a molecular mass of 13009 Dalton. BMP-4 is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: BMP-4 was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives. Solubility: It is recommended to reconstitute the lyophilized BMP-4 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability: Lyophilized BMP-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution BMP-4 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Lys-His-His. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Applications: 1.Molecular standard (Western, ELISA) in studying secreted BMP-4 2.Preparing antibodies for BMP-4 monomer. 3.Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.每孔加3H-TdR 0.5μCi/50μl ↓10h 用多头细胞收集仪收获于玻璃纤维滤纸上 ↓ 干燥后移入液闪瓶中，加入1ml闪烁液，于液闪仪中测β射线的cpm值. 计算： 1. 根据cpm值，将能使7TD1细胞3H-TdR掺入cpm值达到大值50%的IL-6的活性定为1u/ml. 2. 以指数(SI)表示. ㈢ 试剂和器材 1. IL-6依赖的7TD1细胞系 2. 10%FCS-RPMI1640及无FCS的RPMI1640 3. 3H-TdR 4. IL-6 5. PPO,POPOP,. 6. 9999型玻璃纤维滤纸. 7. 96孔平底板 8. 多头细胞收集仪 9. CO2孵箱 10. β液闪计数仪

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